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Image Search Results
Journal: Biology
Article Title: Folate Attenuates Ulcerative Colitis via PI3K/AKT/NF-κB/MLCK Axis Inhibition to Restore Intestinal Barrier Integrity
doi: 10.3390/biology14111573
Figure Lengend Snippet: Colonic transcriptomic analysis after folate administration. ( A ) PCA plot. ( B ) Venn diagram. ( C ) Trend analysis of the DEGs. ( D , E ) Volcano plots. ( F ) KEGG analysis of DEGs. ( G ) Heatmap of DEGs associated with the PI3K/AKT pathway. ( H ) GSEA plots of the PI3K/AKT and NF-κB pathways.
Article Snippet: Primary antibodies used are as follows: GAPDH (1:20,000, Abcam, Cambridge, UK), Occludin (1:2000, Proteintech, Wuhan, China), ZO-1 (1:5000, Proteintech), Claudin-1 (1:1000, Proteintech), Claudin-4 (1:1000, Abcam), E-cadherin (1:2000, CellSignalingTechnology, Danvers, MA, USA), β-catenin (1:1000, CellSignalingTechnology), PI3K (1:5000, Zenbio, Chengdu, China), phos-PI3K (1:1000, CellSignalingTechnology), ERK (1:2000, CellSignalingTechnology), phos-ERK (1:2000, CellSignalingTechnology), AKT (1:2000, Proteintech), NF-κB p65 (1:1000, Proteintech),
Techniques:
Journal: Biology
Article Title: Folate Attenuates Ulcerative Colitis via PI3K/AKT/NF-κB/MLCK Axis Inhibition to Restore Intestinal Barrier Integrity
doi: 10.3390/biology14111573
Figure Lengend Snippet: Folate inhibits the PI3K/AKT/NF-κB/MLCK/MLC2 axis. ( A , B ) Immunoblotting analysis of colonic PI3K, AKT, and NF-κB p65 levels and their phosphorylation states ( n = 3). ( C , D ) Immunoblotting analysis of colonic MLCK, MLC2 and phos-MLC2 ( n = 3). ( E , F ) Immunoblotting analysis of cellular levels of PI3K, AKT, and NF-κB p65 and their phosphorylation states ( n = 3). ( G , H ) Immunoblotting analysis of cellular MLCK, MLC2 and phos-MLC2 ( n = 3). p values are provided in the figures and were determined by one-way ANOVA.
Article Snippet: Primary antibodies used are as follows: GAPDH (1:20,000, Abcam, Cambridge, UK), Occludin (1:2000, Proteintech, Wuhan, China), ZO-1 (1:5000, Proteintech), Claudin-1 (1:1000, Proteintech), Claudin-4 (1:1000, Abcam), E-cadherin (1:2000, CellSignalingTechnology, Danvers, MA, USA), β-catenin (1:1000, CellSignalingTechnology), PI3K (1:5000, Zenbio, Chengdu, China), phos-PI3K (1:1000, CellSignalingTechnology), ERK (1:2000, CellSignalingTechnology), phos-ERK (1:2000, CellSignalingTechnology), AKT (1:2000, Proteintech), NF-κB p65 (1:1000, Proteintech),
Techniques: Western Blot, Phospho-proteomics